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Increasing evidence indicates that maternal malnutrition leads to decreased female fertility and deregulated metabolic homeostasis in offspring, and these performances may be related to the high level of non-esterified fatty acids (NEFAs) in the maternal follicular fluid (FF), but the mechanisms remain unclear. This study is aiming to explore the effect of abnormal NEFAs level on porcine oocyte maturation and early embryo development. We used porcine monolayer of granulosa cells and cumulus-oocyte complexes co-cultured system to mimic the follicular microenvironment. 1% and 10% of porcine FF (PFF) were used to find whether we can more effectively explore the effect of NEFAs by diminishing the interference of PFF. With 1% PFF, the maturation rate was 73.20 % (n=106) which was not significantly different compared to 10% PFF group (86.57 %, n=163), and the blastocyst rate was also similar (27.23 …