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Previous meta-analyses have examined gender differences in people's attitudes toward lesbian/gay sexualities, finding that, overall, men hold more homonegative attitudes than women. Bisexuality scholars have suggested a similar gender difference in attitudes toward bisexuality. This study is the first meta-analysis to provide a comprehensive quantitative synthesis of gender differences in attitudes toward bisexual people and bisexuality. We synthesized findings from 61 studies (including 10 unpublished papers) from 1999 to 2022 that reported on 77 samples of 32,010 participants (14,359 men and 17,651 women). Overall, men held more binegative attitudes than women, g= 0.19, 95% CI [0.14, 0.25]. This effect was moderated by target gender: men were more binegative than women when considering male bisexuality, g= 0.27 [0.20, 0.35]; the effect was substantially smaller when considering female bisexuality …