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Many microorganisms are capable of producing exopolysaccharides which shows flocculation and emulsification properties. In the present study, the strains capable of producing were isolated from the abandoned water tank of SK Porwal College, Kamptee, India. All the three strains were characterized and confirmed by 16S rRNA sequencing and were found to be two strains of bacillus subtilis and one of shigella flexneri. All the three culture strains are producing exopolysaccharides which show profound biotechnological application and also shows flocculation and emulsification properties. The emulsification of kerosene was observed to be around 60% by Bacillus subtilis (MH192392), 40% by shigella flexneri (MH192393) and around 42% by bacillus subtilis (MH646667). Bacillus subtilis (MH192392) showed 55.6% flocculating activity, shigella flexneri (MH192393) and bacillus subtilis (MH646667) showed 88.7% and 95.6% flocculating activity respectively.