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The release of anthropogenic chemicals to streams, stemming from contaminated sites, agriculture, urban sources, or accidental input, represents a significant threat to water resources and thus the health of humans and aquatic ecosystems. Predicting the transport and fate of chemicals is critical to quantifying contaminant concentrations and developing environmental quality standards (EQS). Tracer tests are a well-established tool for such hydrological investigations in various aquatic systems. In stream settings, the experiments have predominantly investigated longitudinal mixing and flow velocities by measuring the tracer concentration in a few discrete locations; few studies have focused on the transverse mixing properties. Recent progress in hyperspectral remote sensing from Unoccupied Aerial Systems (UAS) allows advancing the two-dimensional monitoring of tracer tests, by mapping the tracer concentration …