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Background: Cementum formation is considered to be a critical event for successful regeneration of periodontal tissues. Cementoblasts share many characteristics with osteoblasts. Prostaglandin E₂ (PGE₂) is an important local factor in bone metabolism. Although the effects of PGE₂ on osteoblasts are well known, its effects on cementoblasts have not yet been established. We examined the effects of PGE₂ on proliferation and differentiation in a mouse cementoblast cell line, OCCM‐30 cells.

Methods: OCCM‐30 cells were treated with three concentrations of PGE₂ (10, 100, and 1,000 ng/ml). Cell number, alkaline phosphatase (ALP) activity, and expression for mineralization‐related genes were determined. Osteoprotegerin (OPG) and receptor activator of nuclear factor‐kappa B (NF‐κB) ligand (RANKL) expression were also examined by real‐time polymerase chain reaction (RT‐PCR) and enzyme‐linked …