Authors
Bo Meng, Steven A Kemp, Guido Papa, Rawlings Datir, Isabella ATM Ferreira, Sara Marelli, William T Harvey, Spyros Lytras, Ahmed Mohamed, Giulia Gallo, Nazia Thakur, Dami A Collier, Petra Mlcochova, Samuel C Robson, Nicholas J Loman, Thomas R Connor, Tanya Golubchik, Rocio T Martinez Nunez, Catherine Ludden, Sally Corden, Ian Johnston, David Bonsall, Colin P Smith, Ali R Awan, Giselda Bucca, M Estee Torok, Kordo Saeed, Jacqui A Prieto, David K Jackson, William L Hamilton, Luke B Snell, Catherine Moore, Ewan M Harrison, Sonia Goncalves, Derek J Fairley, Matthew W Loose, Joanne Watkins, Rich Livett, Samuel Moses, Roberto Amato, Sam Nicholls, Matthew Bull, Darren L Smith, Jeff Barrett, David M Aanensen, Martin D Curran, Surendra Parmar, Dinesh Aggarwal, James G Shepherd, Matthew D Parker, Sharon Glaysher, Matthew Bashton, Anthony P Underwood, Nicole Pacchiarini, Katie F Loveson, Kate E Templeton, Cordelia F Langford, John Sillitoe, Thushan I de Silva, Dennis Wang, Dominic Kwiatkowski, Andrew Rambaut, Justin O’Grady, Simon Cottrell, Matthew TG Holden, Emma C Thomson, Husam Osman, Monique Andersson, Anoop J Chauhan, Mohammed O Hassan-Ibrahim, Mara Lawniczak, Alex Alderton, Meera Chand, Chrystala Constantinidou, Meera Unnikrishnan, Alistair C Darby, Julian A Hiscox, Steve Paterson, Inigo Martincorena, Erik M Volz, Andrew J Page, Oliver G Pybus, Andrew R Bassett, Cristina V Ariani, Michael H Spencer Chapman, Kathy K Li, Rajiv N Shah, Natasha G Jesudason, Yusri Taha, Martin P McHugh, Rebecca Dewar, Aminu S Jahun, Claire McMurray, Sarojini Pandey, James P McKenna, Andrew Nelson, Gregory R Young, Clare M McCann, Scott Elliott, Hannah Lowe, Ben Temperton, Sunando Roy, Anna Price, Sara Rey, Matthew Wyles, Stefan Rooke, Sharif Shaaban, Mariateresa de Cesare, Laura Letchford, Siona Silveira, Emanuela Pelosi, Eleri Wilson-Davies, Myra Hosmillo, Áine O’Toole, Andrew R Hesketh, Richard Stark, Louis du Plessis, Chris Ruis, Helen Adams, Yann Bourgeois, Stephen L Michell, Dimitris Grammatopoulos, Jonathan Edgeworth, Judith Breuer, John A Todd, Christophe Fraser, David Buck, Michaela John, Gemma L Kay, Steve Palmer, Sharon J Peacock, David Heyburn, Danni Weldon, Esther Robinson, Alan McNally, Peter Muir, Ian B Vipond, John Boyes, Venkat Sivaprakasam, Tranprit Salluja, Samir Dervisevic, Emma J Meader, Naomi R Park, Karen Oliver, Aaron R Jeffries, Sascha Ott, Ana da Silva Filipe, David A Simpson, Chris Williams, Jane AH Masoli
Publication date
2021/6/29
Journal
Cell reports
Volume
35
Issue
13
Publisher
Elsevier
Description
We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer antibody escape. In vitro, we show that, although ΔH69/V70 itself is not an antibody evasion mechanism, it increases infectivity associated with enhanced incorporation of cleaved spike into virions. ΔH69/V70 is able to partially rescue infectivity of spike proteins that have acquired N439K and Y453F escape mutations by increased spike incorporation. In addition, replacement of the H69 and V70 residues in the Alpha variant B.1.1.7 spike (where ΔH69/V70 occurs naturally) impairs spike incorporation and entry efficiency of the B.1.1.7 spike pseudotyped virus. Alpha variant B.1.1.7 spike mediates faster kinetics of cell-cell fusion …
Scholar articles
Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B. 1.1. 7
B Meng, SA Kemp, G Papa, R Datir, IATM Ferreira… - Cell reports, 2021