Authors
André L Vettore, Felipe R da Silva, Edson L Kemper, Glaucia M Souza, Aline M da Silva, Maria Inês T Ferro, Flavio Henrique-Silva, Éder A Giglioti, Manoel VF Lemos, Luiz L Coutinho, Marina P Nobrega, Helaine Carrer, Suzelei C França, Maurício Bacci, Maria Helena S Goldman, Suely L Gomes, Luiz R Nunes, Luis EA Camargo, Walter J Siqueira, Marie-Anne Van Sluys, Otavio H Thiemann, Eiko E Kuramae, Roberto V Santelli, Celso L Marino, Maria LPN Targon, Jesus A Ferro, Henrique CS Silveira, Danyelle C Marini, Eliana GM Lemos, Claudia B Monteiro-Vitorello, José HM Tambor, Dirce M Carraro, Patrícia G Roberto, Vanderlei G Martins, Gustavo H Goldman, Regina C de Oliveira, Daniela Truffi, Carlos A Colombo, Magdalena Rossi, Paula G de Araujo, Susana A Sculaccio, Aline Angella, Marleide MA Lima, Vicente E de Rosa, Fábio Siviero, Virginia E Coscrato, Marcos A Machado, Laurent Grivet, Sonia MZ Di Mauro, Francisco G Nobrega, Carlos FM Menck, Marilia DV Braga, Guilherme P Telles, Frank AA Cara, Guilherme Pedrosa, João Meidanis, Paulo Arruda
Publication date
2003/12/1
Journal
Genome research
Volume
13
Issue
12
Pages
2725-2735
Publisher
Cold Spring Harbor Lab
Description
To contribute to our understanding of the genome complexity of sugarcane, we undertook a large-scale expressed sequence tag (EST) program. More than 260,000 cDNA clones were partially sequenced from 26 standard cDNA libraries generated from different sugarcane tissues. After the processing of the sequences, 237,954 high-quality ESTs were identified. These ESTs were assembled into 43,141 putative transcripts. Of the assembled sequences, 35.6% presented no matches with existing sequences in public databases. A global analysis of the whole SUCEST data set indicated that 14,409 assembled sequences (33% of the total) contained at least one cDNA clone with a full-length insert. Annotation of the 43,141 assembled sequences associated almost 50% of the putative identified sugarcane genes with protein metabolism, cellular communication/signal transduction, bioenergetics, and stress responses …
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Scholar articles
AL Vettore, FR da Silva, EL Kemper, GM Souza… - Genome research, 2003