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Antioxidants are bioactive components used to relieve the detrimental effects of oxidative stress caused by the presence of free radicals. These valuable compounds are naturally available in medicinal plants. The present study aims to investigate the influence of two independent variables, namely temperature (oC) and time (hours) on the extraction yield of phenolic compounds, flavonoids and antioxidant activity from garlic, oregano, and parsley. The optimized conditions for the extraction of bioactive components from medicinal plants were determined using two-factor central composite design (CCD) combined with response surface methodology (RSM). The order of experiments was completely randomized using central composite design with five (5) centre points. All experimental data was analyzed using "Design Expert" software (Design-Expert 7.0.0 Trial, State-Ease Inc., Minneapolis MN, USA). A second …