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Affective materials have always been an important factor in improving the efficiency of affect related research for their ecological validity. In order to achieve high ecological validity in emotion elicitation, this paper proposed a novel Affective Virtual Reality System (AVRS) by utilizing the immersion, privacy and design flexibility of virtual reality. Design elements including subject features, sound features, motion features and color features were extracted by referring to the referenced emotion materials, art works and the existing VR scenes. Affective VR scenes were then designed by Unreal Engine 4.12 and their effectiveness was validated by Self-Assessment Manikin (SAM). Furthermore, arousal was used as an indicator to compare the difference of ecological validity between VR and video emotional materials with an intergroup experiment through Electroencephalography (EEG), Heart Rate (HR), Galvanic Skin …