Authors
Josep M Llovet, Yingbei Chen, Elisa Wurmbach, Sasan Roayaie, M Isabel Fiel, Myron Schwartz, Swan N Thung, Gregory Khitrov, Weijia Zhang, Augusto Villanueva, Carlo Battiston, Vincenzo Mazzaferro, Jordi Bruix, Samuel Waxman, Scott L Friedman
Publication date
2006/12/1
Journal
Gastroenterology
Volume
131
Issue
6
Pages
1758-1767
Publisher
WB Saunders
Description
Background & Aims
Small liver nodules ∼2 cm are difficult to characterize by radiologic or pathologic examination. Our aim was to identify a molecular signature to diagnose early hepatocellular carcinoma (HCC).
Methods
The transcriptional profiles of 55 candidate genes were assessed by quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) in 17 dysplastic nodules (diameter, 10 mm) and 20 early HCC (diameter, 18 mm) from HCV cirrhotic patients undergoing resection/transplantation and 10 nontumoral cirrhotic tissues and 10 normal liver tissues. Candidate genes were confirmed by quantitative RT-PCR in 20 advanced HCCs and by immunohistochemistry in 75 samples and validated in an independent set of 29 samples (dysplastic nodules [10] and small HCC [19; diameter, 20 mm]).
Results
Twelve genes were significantly, differentially expressed in early HCCs compared with …
Total citations
Scholar articles
JM Llovet, Y Chen, E Wurmbach, S Roayaie, MI Fiel… - Gastroenterology, 2006