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Competence of the bovine embryo to establish pregnancy can be enhanced by treatment with colony stimulating factor 2 (CSF2) from Days 5 to 7 post-insemination. The mechanism is unknown but could involve developmental reprogramming. Here, we questioned whether treatment with CSF2 from Days 5 to 7 alters growth, interferon-τ (IFNT) secretion, and the methylome of extra-embryonic membranes (EEM) at Day 15. In vitro-produced embryos were treated with either 0 or 10 ng mL–1 bovine CSF2 from Days 5 to 7. Expanded blastocysts were transferred into synchronized recipients. On Day 15, conceptuses were recovered by flushing the uterus and sex determined by PCR (n = 4 males and 4 females per treatment). Statistical analysis of IFNT and length was performed using analysis of variance with the general linear models procedure of SAS (SAS Institute Inc., Cary, NC, USA). There was a sex × …