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Two experiments were conducted to determine whether addition of hyaluronan to culture medium could improve survival of bovine embryos after vitrification or following embryo transfer. In Experiment 1, embryos were produced in vitro and cultured for 7 days in modified synthetic oviductal fluid (SOF) containing one of four concentrations of hyaluronan (0, 0.1, 0.5, or 1mg/mL), with or without 4mg/mL of bovine serum albumin (BSA). On Day 7 after insemination, blastocysts and expanded blastocysts were vitrified using open-pulled straws. At a concentration of 1mg/mL, hyaluronan increased (P<0.05) the percentage of oocytes that were blastocysts and re-expansion rate at 24h after warming. At 0.5mg/mL, hyaluronan tended (P<0.10) to increase re-expansion rate at 48h after warming and increased (P<0.05) embryo hatching rate at 24 and 72h. Treatment with BSA caused a slight reduction in cleavage rate (P<0.05 …