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We have carried out a field trial in cattle to study the effect of the interval between the onset of estrus and AI on sex ratio and fertility. Data were obtained from 716 cows that had been inseminated at different times between 8 and 44h from the visual detection of estrus. Before analyzing the data, it was grouped in three intervals considering the time between estrus onset and AI (8–18, 18–30, and ≥30h). Our results show that the percentage of calved females (73.05%) is significantly superior for early inseminations (8–18h), and it decreases 1.85% per hour from the onset of estrus. Delayed AIs (≥30h) produce a significant deviation of the sex ratio towards the males (72.06%); nevertheless, fertility (percentage of successful pregnancies) diminishes significantly, from 66.19% (8–18h) to 45.35% (≥30h). In conclusion, variations in the interval between the onset of estrus and AI modify sex ratio. However, we must …