Authors
PA Larsen, CR Campbell, AD Yoder
Publication date
2014/11
Journal
Molecular Ecology Resources
Volume
14
Issue
6
Pages
1198-1209
Description
High‐throughput sequencing platforms are generating massive amounts of genomic data from nonmodel species, and these data sets are valuable resources that can be mined to advance a number of research areas. An example is the growing amount of transcriptome data that allow for examination of gene expression in nonmodel species. Here, we show how publicly available transcriptome data from nonmodel primates can be used to design novel research focused on immunogenomics. We mined transcriptome data from the world's most endangered group of primates, the lemurs of Madagascar, for sequences corresponding to immunoglobulins. Our results confirmed homology between strepsirrhine and haplorrhine primate immunoglobulins and allowed for high‐throughput sequencing of expressed antibodies (Ig‐seq) in Coquerel's sifaka (Propithecus coquereli). Using both Pacific Biosciences RS and Ion …
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