Authors
Rosa M Xicola, Xavier Llor, Elisenda Pons, Antoni Castells, Cristina Alenda, Virgínia Piñol, Montserrat Andreu, Sergi Castellví-Bel, Artemio Payá, Rodrigo Jover, Xavier Bessa, Anna Girós, José M Duque, David Nicolás-Pérez, Ana M Garcia, Joaquin Rigau, Miquel A Gassull
Publication date
2007/2/7
Journal
Journal of the national cancer institute
Volume
99
Issue
3
Pages
244-252
Publisher
Oxford University Press
Description
Background
Colorectal tumors caused by failure of the DNA mismatch repair system commonly show microsatellite instability. Our goals were to compare the performance of two panels of markers (a panel previously recommended by the National Cancer Institute [NCI] and a pentaplex of mononucleotide repeats) and to devise the simplest diagnostic strategy for identification of patients with colorectal cancer characterized by defects in mismatch repair.
Methods
We recruited 1058 patients who were newly diagnosed with colorectal cancer. DNA from fresh-frozen and paraffin-embedded tumors was tested for microsatellite instability, using the NCI-recommended panel of microsatellite markers and the pentaplex panel of mononucleotide repeats, respectively, as templates for polymerase chain reactions (PCRs). Microsatellite instability in fresh-frozen tumors was also assessed …
Total citations
Scholar articles
RM Xicola, X Llor, E Pons, A Castells, C Alenda… - Journal of the national cancer institute, 2007