Authors
Thomas Broquet, Laura Berset-Braendli, Guillaume Emaresi, Luca Fumagalli
Publication date
2007/4
Journal
Conservation Genetics
Volume
8
Pages
509-511
Publisher
Kluwer Academic Publishers
Description
Buccal swabs have recently been used as a minimally invasive sampling method in genetic studies of wild populations, including amphibian species. Yet it is not known to date what is the level of reliability for microsatellite genotypes obtained using such samples. Allelic dropout and false alleles may affect the genotyping derived from buccal samples. Here we quantified the success of microsatellite amplification and the rates of genotyping errors using buccal swabs in two amphibian species, the Alpine newt Triturus alpestris and the Green tree frog Hyla arborea, and we estimated two important parameters for downstream analyses, namely the number of repetitions required to achieve typing reliability and the probability of identity among genotypes. Amplification success was high, and only one locus tested required two to three repetitions to achieve reliable genotypes, showing that buccal swabbing is a …
Total citations
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Scholar articles
T Broquet, L Berset-Braendli, G Emaresi, L Fumagalli - Conservation Genetics, 2007