Authors
Camillo Ricordi, Derek WR Gray, Bernhard J Hering, Dixon B Kaufman, Garth L Warnock, Norman M Kneteman, Stephen P Lake, Nicholas JM London, Carlo Socci, Rodolfo Alejandro, Yijun Zeng, David W Scharp, Giorgio Viviani, Luca Falqui, Andreas Tzakis, Reinhard G Bretzel, Konrad Federlin, Guido Pozza, Roger FL James, Ray V Rajotte, Valerio Di Carlo, Peter J Morris, David ER Sutherland, Thomas E Starzl, Daniel H Mintz, Paul E Lacy
Publication date
1990/7
Journal
Acta diabetologia latina
Volume
27
Pages
185-195
Publisher
Springer-Verlag
Description
Recent progress in islet isolation from the pancreas of large mammals including man, accentuated the need for the development of precise and reproducible techniques to assess islet yield. In this report both quantitative and qualitative criteria for islet isolation assessment were discussed, the main topics being the determination of number, volume, purity, morphologic integrity andin vitro andin vivo function tests of the final islet preparations. It has been recommended that dithizone should be used as a specific stain for immediate detection of islet tissue making it possible to estimate both the total number of islets (dividing them into classes of 50 µ diameter range increments) and the purity of the final preparation. Appropriate morphological assessment should include confirmation of islet identification, assessment of the morphological integrity and of the purity of the islet preparation. The use of fluorometric …
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Scholar articles
C Ricordi, DWR Gray, BJ Hering, DB Kaufman… - Acta diabetologia latina, 1990