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Misorientation data from Electron Backscatter Diffraction (EBSD) is often used to identify strain localisation and quantify plastic strain at the microstructural scale. However, the exact relationship between local plastic strain and misorientation and how it changes at the grain and sub-grain level has not been studied in detail. We have used high resolution digital image correlation (HRDIC) to measure plastic strain at the sub-micron scale on the surface of a nickel superalloy strained to 2%. The strain values have been correlated to different misorientation measures at the grain and subgrain scale, over several hundreds of grains. We show that although the grain mean plastic strain is positively correlated to the lattice misorientation, there is a large scatter in the correlation, which depends on the misorientation measure used. There is also essentially no correlation between the magnitude of grain strain and grain …