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Plant growth-promoting rhizobacterial strains belonging to fluorescent pseudomonads were isolated from the rhizosphere of rice and sugarcane. Among 40 strains that were confirmed as Pseudomonas fluorescens, 18 exhibited strong antifungal activity against Rhizoctonia bataticola and Fusarium oxysporum, mainly through the production of antifungal metabolites. Genotyping of these P. fluorescens strains was made by PCR–RAPD analysis, since differentiation by biochemical methods was limited.