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Depending on conditions in the refining process, soybean oil deodorizer distillate (SODD) in Taiwan typically contains about 45% free fatty acids (FFAs) and 20% triacylglycerols (TAGs). Bioactive compounds such as tocopherols, free phytosterols, fatty acid steryl esters (FASEs) and squalene also make up a significant portion of SODD. In the present work, a modified soxhlet extraction and silica gel column chromatography were employed to isolate and purify squalene from SODD. The goal of this work was to obtain a quantitative analysis of the separation processes and to assess the feasibility of this method to isolate the constituent compounds. Here, a modified soxhlet extraction was employed for the efficient separation of FASEs and squalene into one fraction, and tocopherols, free phytosterols, TAGs and FFAs into another fraction. Starting with SODD that contains 3.91% FASEs, 1.83% squalene, 6.40 …