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This paper describes an experimental study of using tyre pyrolysis oil (TPO) obtained from waste automobile tyres by vacuum pyrolysis method, as a fuel in diesel engine. In this work, performance and emission parameters of a single cylinder water cooled diesel engine running on TPO diesel reference fuel (RF) blends in steps of 20% on volume basis of TPO, viz. TPO20 up to TPO70 were used as fuels and the results compared with diesel operation. Results indicated that reliable operation can be achieved up to 70% of TPO diesel blends. Thermal efficiencies were lower compared to diesel operation. Higher smoke, HC and CO emissions were recorded in the experimentation. Oil sticking was occasionally found on the nozzle stem and sac. There was no corrosion in the injection system after running the engine with TPO–RF blends.