Authors
Haixiao Gao, Jayati Sengupta, Mikel Valle, Andrei Korostelev, Narayanan Eswar, Scott M Stagg, Patrick Van Roey, Rajendra K Agrawal, Stephen C Harvey, Andrej Sali, Michael S Chapman, Joachim Frank
Publication date
2003/6/13
Journal
Cell
Volume
113
Issue
6
Pages
789-801
Publisher
Elsevier
Description
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
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Scholar articles
H Gao, J Sengupta, M Valle, A Korostelev, N Eswar… - Cell, 2003