Authors
Robert M Resnick, Marion TE Cornelissen, Deann K Wright, Greg H Eichinger, Howard S Fox, Jan ter Schegget, M Michele Manos
Publication date
1990/9/19
Journal
JNCI: Journal of the National Cancer Institute
Volume
82
Issue
18
Pages
1477-1484
Publisher
Oxford University Press
Description
We developed a polymerase chain reaction DNA amplification system using two distinct consensus oligonucleotide primer sets for the improved detection and typing of a broad spectrum of human genital papillomavirus (HPV) sequences, including those of novel viruses. The system incorporates one primer set designed to amplify a highly conserved L1 domain and a second primer set designed to amplify a domain within the E6 gene. We used this system to analyze 48 fixed, paraffin-embedded tissue sections (41 specimens from 33 cervical carcinomas, four normal cervical tissues, and several control tissues) for the presence of HPV DNA. HPV sequences were detected in all carcinoma samples and none of the control samples. Hybridization analyses showed that the results obtained with the two amplification schemes concurred completely. This approach allowed rapid confirmation of typing results and may …
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Scholar articles
RM Resnick, MTE Cornelissen, DK Wright… - JNCI: Journal of the National Cancer Institute, 1990