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The present study investigated neuromuscular adaptations between same-session combined strength and endurance training with 2 loading orders and different day combined training over 24 weeks. 56 subjects were divided into different day (DD) combined strength and endurance training (4–6 d^·wk^-1) and same-session combined training: endurance preceding strength (E+S) or vice versa (S+E) (2–3 d^·wk^-1). Dynamic and isometric strength, EMG, voluntary activation, muscle cross-sectional area and endurance performance were measured. All groups increased dynamic one-repetition maximum (p<0.001; DD 13±7%, E+S 12±9% and S+E 17±12%) and isometric force (p<0.05–0.01), muscle cross-sectional area (p<0.001) and maximal power output during cycling (p<0.001). DD and S+E increased voluntary activation during training (p<0.05–0.01). In E+S no increase in voluntary activation was detected after 12 …