Authors
Alexandra F Santos, Abdel Douiri, Natalia Bécares, Shih-Ying Wu, Alick Stephens, Suzana Radulovic, Susan MH Chan, Adam T Fox, George Du Toit, Victor Turcanu, Gideon Lack
Publication date
2014/9/1
Journal
Journal of allergy and clinical immunology
Volume
134
Issue
3
Pages
645-652
Publisher
Mosby
Description
Background
Most of the peanut-sensitized children do not have clinical peanut allergy. In equivocal cases, oral food challenges (OFCs) are required. However, OFCs are laborious and not without risk; thus, a test that could accurately diagnose peanut allergy and reduce the need for OFCs is desirable.
Objective
To assess the performance of basophil activation test (BAT) as a diagnostic marker for peanut allergy.
Methods
Peanut-allergic (n = 43), peanut-sensitized but tolerant (n = 36) and non–peanut-sensitized nonallergic (n = 25) children underwent skin prick test (SPT) and specific IgE (sIgE) to peanut and its components. BAT was performed using flow cytometry, and its diagnostic performance was evaluated in relation to allergy versus tolerance to peanut and validated in an independent population (n = 65).
Results
BAT in peanut-allergic children showed a peanut dose-dependent upregulation of CD63 and …
Scholar articles
AF Santos, A Douiri, N Bécares, SY Wu, A Stephens… - Journal of allergy and clinical immunology, 2014