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Data assimilation techniques integrating crop growth models and remote sensing technologies offer a feasible approach for large-scale crop yield estimation. Previous research has primarily focused on either recalibrate the uncertain model parameters or updating model state variables independently using remotely sensed observations. In this study, we developed a two-step inference algorithm that couples the parameter inference and the state update, to solve the joint posterior distribution of uncertain parameters and state variables given the remote sensing observations. An Observing Simulation System (OSS) experiment was first performed based on the WOFOST crop model to validate the effectiveness of the parameter inference method. The results indicate that, the parameter inference method successfully improved the estimation of different types of model parameters and enhanced yield estimation …