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We have set forth a design strategy for creating biomimetic materials that direct the formation of tissue surrounding implants or regeneration within porous scaffolds. Our studies have established that heterogeneous mimetic peptide surfaces (MPS) containing both the ‐RGD‐ (cell‐binding) and ‐FHRRIKA‐(putative heparin‐binding) peptides, unique to BSP, in the ratio of 75:25 (MPS II) or 50:50 (MPS III) proved to be more biologically relevant and specific for RCO cell function. The initial response of human osteoblast‐like cells to these surfaces was mediated by the collagen (α₂β₁) and vitronectin receptors (α_vβ₃), whereas the vitronectin receptor alone dominated longer‐term events (> 30 min). MPS II and III surfaces enhanced cell spreading and long‐term events such as mineralization of the extracellular matrix compared to homogenous peptide surfaces and controls. Furthermore, extensive mineralization of the …