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2. MATERIALS AND METHODS 2.1 Experimental set-up Two identical laboratory set-ups were applied for the removal of hydrogen sulfide. Both of columns were inoculated sulfur oxidizing bacteria. One reactor was running with deionized water, the other one were operated with secondary effluent water from waste water treatment plant (Yong-in city, Korea). Biotrickling filter construction and operating conditions are shown in Table 1 and Figure 1. H2S was introduced by reaction of HCl and solution of Na2S. H2S concentrations ranging from 0 to 200 ppm were obtained by changing the Na2S concentration and/or the dripping rate.

2.2 Microorganisms and cultivation Sulfide oxidizing bacteria were enriched in initially autoclaved thiosulfate media at 150rpm 280C. The composition of the liquid medium used was (in g/L): KH2PO4, 2; K2HPO4, 2; NH4Cl, 0.4; MgCl2. 6H2O, 0.2; FeSO4. 7H2O, 0.01; Na2S2O3. 5H2O, 8 …