Authors
G Danscher
Publication date
1984/7
Journal
Histochemistry
Volume
81
Issue
4
Pages
331-335
Publisher
Springer-Verlag
Description
The autometallographic procedure represents a new technique that can substitute for the normal methods of physical development (PD). The physical developer (a solution of reducing substance, silver salt and protection colloid) is replaced by a photographic emulsion and chemical developer. Accumulations of gold, silver, metal sulphides and metal selenides can be amplified by the present technique.
Tissue sections placed on glass slides are covered by a silver bromide containing emulsion, dried and exposed to a chemical developer. After development the emulsion is either removed or cleared and the sections are counterstained and embedded.
The autometallographic procedure can also be applied to ultrathin sections.
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