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Extracellular polymeric substances (EPS), especially polysaccharides, are viewed as the major foulants during filtration of biological feeds. Numerous attempts to quantify their influence have been made, however, few studies investigated their actual fouling mechanisms or determined if model polysaccharides are representative of real EPS. Here, crossflow filtration of model polysaccharides xanthan, actigum CS 11 and glucan was carried out. The fate of polysaccharides was determined through UV absorbance, light scattering, ATR-FTIR and viscosity measurements, and the results were analysed using the resistance-in-series model and classical filtration laws. The cake filtration model appears to fit the entire range of the ultrafiltration data for polysaccharide solutions at a low concentration of 20mg/L. At higher concentrations, identification of the predominant fouling mechanism was difficult. This indicates that the …