Authors
Ze-Xiong Xie, Bing-Zhi Li, Leslie A Mitchell, Yi Wu, Xin Qi, Zhu Jin, Bin Jia, Xia Wang, Bo-Xuan Zeng, Hui-Min Liu, Xiao-Le Wu, Qi Feng, Wen-Zheng Zhang, Wei Liu, Ming-Zhu Ding, Xia Li, Guang-Rong Zhao, Jian-Jun Qiao, Jing-Sheng Cheng, Meng Zhao, Zheng Kuang, Xuya Wang, J Andrew Martin, Giovanni Stracquadanio, Kun Yang, Xue Bai, Juan Zhao, Meng-Long Hu, Qiu-Hui Lin, Wen-Qian Zhang, Ming-Hua Shen, Si Chen, Wan Su, En-Xu Wang, Rui Guo, Fang Zhai, Xue-Jiao Guo, Hao-Xing Du, Jia-Qing Zhu, Tian-Qing Song, Jun-Jun Dai, Fei-Fei Li, Guo-Zhen Jiang, Shi-Lei Han, Shi-Yang Liu, Zhi-Chao Yu, Xiao-Na Yang, Ken Chen, Cheng Hu, Da-Shuai Li, Nan Jia, Yue Liu, Lin-Ting Wang, Su Wang, Xiao-Tong Wei, Mei-Qing Fu, Lan-Meng Qu, Si-Yu Xin, Ting Liu, Kai-Ren Tian, Xue-Nan Li, Jin-Hua Zhang, Li-Xiang Song, Jin-Gui Liu, Jia-Fei Lv, Hang Xu, Ran Tao, Yan Wang, Ting-Ting Zhang, Ye-Xuan Deng, Yi-Ran Wang, Ting Li, Guang-Xin Ye, Xiao-Ran Xu, Zheng-Bao Xia, Wei Zhang, Shi-Lan Yang, Yi-Lin Liu, Wen-Qi Ding, Zhen-Ning Liu, Jun-Qi Zhu, Ning-Zhi Liu, Roy Walker, Yisha Luo, Yun Wang, Yue Shen, Huanming Yang, Yizhi Cai, Ping-Sheng Ma, Chun-Ting Zhang, Joel S Bader, Jef D Boeke, Ying-Jin Yuan
Publication date
2017/3/10
Journal
Science
Volume
355
Issue
6329
Pages
eaaf4704
Publisher
American Association for the Advancement of Science
Description
INTRODUCTION
The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both a synthetic yeast chromosome arm (synIXR) and the entirely synthetic chromosome (synIII) function with high fitness in yeast. For designer genome synthesis projects, precise engineering of the physical sequence to match the specified design is important for the systematic evaluation of underlying design principles. Yeast can maintain nuclear chromosomes as rings, occurring by chance at repeated sequences, although the cyclized format is unfavorable in meiosis given the possibility of dicentric chromosome formation from meiotic recombination. Here, we describe the de novo synthesis of synthetic yeast chromosome V (synV) in the “Build-A-Genome China” course, perfectly matching the designer sequence and bearing loxPsym sites, distinguishable …
Scholar articles
ZX Xie, BZ Li, LA Mitchell, Y Wu, X Qi, Z Jin, B Jia… - Science, 2017