Authors
Ramon Massana, Angélique Gobet, Stéphane Audic, David Bass, Lucie Bittner, Christophe Boutte, Aurélie Chambouvet, Richard Christen, Jean‐Michel Claverie, Johan Decelle, John R Dolan, Micah Dunthorn, Bente Edvardsen, Irene Forn, Dominik Forster, Laure Guillou, Olivier Jaillon, Wiebe HCF Kooistra, Ramiro Logares, Frédéric Mahé, Fabrice Not, Hiroyuki Ogata, Jan Pawlowski, Massimo C Pernice, Ian Probert, Sarah Romac, Thomas Richards, Sébastien Santini, Kamran Shalchian‐Tabrizi, Raffaele Siano, Nathalie Simon, Thorsten Stoeck, Daniel Vaulot, Adriana Zingone, Colomban de Vargas
Publication date
2015/10
Journal
Environmental microbiology
Volume
17
Issue
10
Pages
4035-4049
Description
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico‐, nano‐ and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta‐diversity analyses split the protist community structure into three main clusters: picoplankton‐nanoplankton‐dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together, while communities from the same site but different seasons were unrelated. Both DNA and RNA‐based surveys provided similar relative abundances …
Scholar articles
R Massana, A Gobet, S Audic, D Bass, L Bittner… - Environmental microbiology, 2015