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The field experiments on" Screening of Brassica genotypes against mustard aphid, Lipaphis erysimi Kalt." was conducted at Students' Instructional Farm of CS Azad University of Agriculture & Technology, Kanpur during Rabi 2018-19 and 2019-20. Sixty Brassica genotypes were evaluated on the basis of the Aphid Infestation Index (AII)('0-5'scale) twice first at 50% flowering stage and second at the pod setting stage. The population of mustard aphids was observed in 60 genotypes taken under screening to see the performance of genotypes based on the level of infestation of mustard aphids. Based on AII the genotypes viz., Kanti, Kranti, Jagannath, Krishna, Maya, Pusa Bahar, and Pusa Agrani (Sej 2) were found to be highly resistant. Based on the aphid population the genotypes viz., Kanti, Kranti, Jagannath, Geeta, and Maya were found to be highly resistant.