Authors
Margaritis Avgeris, Georgios Koutalellis, Emmanuel G Fragoulis, Andreas Scorilas
Publication date
2008/10/1
Journal
Clinical biochemistry
Volume
41
Issue
14-15
Pages
1140-1149
Publisher
Elsevier
Description
BACKGROUND
L-Dopa decarboxylase (DDC) is a pyridoxal 5′-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP).
METHODS
Total RNA was isolated from 118 tissue specimens from benign prostate hyperplasia (BPH) and CaP patients and a highly sensitive quantitative real-time RT-PCR (qRT-PCR) method for DDC mRNA quantification has been developed using the SYBR Green® chemistry. LNCaP prostate cancer cell line was used as a calibrator and GAPDH as a housekeeping gene.
RESULTS
DDC was found to be overexpressed, at the mRNA level, in the specimens from prostate cancer patients, in comparison to those from benign prostate hyperplasia patients (p<0.001). Logistic regression and ROC analysis have …
Total citations
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Scholar articles
M Avgeris, G Koutalellis, EG Fragoulis, A Scorilas - Clinical biochemistry, 2008