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In the subtropics, the effects of macrophytes on trophic interactions are more complex than in temperate lakes. Fish, particularly the smallest species and individuals, aggregate in high numbers in the vegetation, and a strong predation pressure on Zooplankton by shrimps and invertebrates, such as Chaoborus, can occur in these systems. We studied seasonal and diel changes in Zooplankton and their potential predators (both fish and invertebrates) and physical and chemical characteristics among open water and vegetated habitats (emergent and submerged plants (SP)), in the subtropical Lake Bianca (34°54’ S; 54°50’ W), a shallow system with an extensive and complex littoral area and high abundance of vertebrate and invertebrate predators on Zooplankton. We found differential horizontal distribution of some zooplankton species under the scenario of high abundance of small omnivorous …