Authors
Caroline Kerbiriou, Caitlin Dickson, Ben Nichols, Michael Logan, Anna Mascellani, Jaroslav Havlik, Richard K Russell, Richard Hansen, Simon Milling, Konstantinos Gerasimidis
Publication date
2024/7/9
Journal
Inflammatory Bowel Diseases
Pages
izae124
Publisher
Oxford University Press
Description
Background
Exclusive enteral nutrition (EEN) is an effective treatment for active Crohn’s disease (CD). This study explored the immunostimulatory potential of a cell-free fecal filtrate and related this with changes in the fecal microbiota and metabolites in children with active CD undertaking treatment with EEN.
Methods
Production of tumor necrosis factor α (TNFα) from peripheral blood mononuclear cells was measured following their stimulation with cell-free fecal slurries from children with CD, before, during, and at completion of EEN. The metabolomic profile of the feces used was quantified using proton nuclear magnetic resonance and their microbiota composition with 16S ribosomal RNA sequencing.
Results
Following treatment with EEN, 8 (72%) of 11 patients demonstrated a reduction in fecal calprotectin (FC) >50% and were subsequently labeled FC …
Scholar articles
C Kerbiriou, C Dickson, B Nichols, M Logan… - Inflammatory Bowel Diseases, 2024