View article

Biophysical cues such as substrate modulus have been shown to influence a variety of cell behaviors. We have determined the elastic modulus of the anterior basement membrane and Descemet’s membrane of the human cornea with atomic force microscopy (AFM). A spherical probe was used with a radius approximating that of a typical cell focal adhesion. Values obtained for the elastic modulus of the anterior basement membrane range from 2 to 15kPa, with a mean of 7.5±4.2kPa. The elastic modulus of Descemet’s membrane was found to be slightly higher than those observed for the anterior basement membrane, with a mean of 50±17.8kPa and a range of 20–80kPa. The topography of Descemet’s membrane has been shown to be similar to that of the anterior basement, but with smaller pore sizes resulting in a more tightly packed structure. This structural difference may account for the observed modulus …