View article

Results: The collagen-dependent T47D cells (pZip and 38V) were able to adhere to the uncoated and the collagen-coated plates; however, the morphology of the cells was improved by plating onto the collagen-coated plates (A). Similarly, the coefficient of variance (CV) was dramatically lower when pZip and R-Ras (38V) cells were seeded on the collagen-coated plates (C) compared to that observed when the cells were seeded on the uncoated plates (B). The inhibitory effect of R-Ras (38V) expression could not be validated when the cell migration assay was performed using uncoated tissue culture plates. However, when the assay was performed by seeding the R-Ras (38V) cells onto collagen-coated plates, the cells were inhibited in their motility; thus validating previously published findings (Reference 1).