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Siloxanes are emerging environmental contaminants that accumulate in wastewater treatment plants, leading to the production of biogas enriched with these compounds and the breakdown of cogeneration equipment. This study aims to evaluate the wastewater treatment by microalgae (Chlorella vulgaris) in simulated environments with different decamethylcyclopentasiloxane (D5) concentrations and analyse the distribution of this compound in four matrices:(i) air;(ii) microalgal biomass;(iii) microalgal suspension; and (iv) water. D5 was detected in the water and microalgal suspension samples from the first day, but not in the last day. The volatilisation of D5 before its adherence to microalgae or the degradation of D5 by microalgae could explain these results.