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In this pilot study three fractions of particulate matter (PM_0.25, PM_2.5-0.25, and PM_10-2.5) were collected in three environments (classroom, home, and outdoors) in a village located nearby an industrial complex. Time-activity pattern of 20 students attending the classroom was obtained, and the dose of particles reaching the children's lungs under actual environmental conditions (i.e. real dose) was calculated via dosimetry model. The highest PM concentrations were reached in the classroom. Simulations showed that heavy intensity outdoor activities played a major role in PM deposition, especially in the upper part of the respiratory tract. The mass of PM_10-2.5 reaching the alveoli was minor, while PM_2.5-0.25 and PM_0.25 apportion for most of the PM mass retained in the lungs. Consequently, PM_2.5-0.25 and PM_0.25 were the only fractions used in two subsequent toxicity assays onto alveolar cells (A549). First, a …