Authors
Ruaa Al-Qazazi, Patricia DA Lima, Sasha Z Prisco, Francois Potus, Asish Dasgupta, Kuang-Hueih Chen, Lian Tian, Rachel ET Bentley, Jeff Mewburn, Ashley Y Martin, Danchen Wu, Oliver Jones, Donald H Maurice, Sebastien Bonnet, Steeve Provencher, Kurt W Prins, Stephen L Archer
Publication date
2022/9/1
Journal
American journal of respiratory and critical care medicine
Volume
206
Issue
5
Pages
608-624
Publisher
American Thoracic Society
Description
Rationale: Pulmonary arterial hypertension (PAH) often results in death from right ventricular failure (RVF). NLRP3 (nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3)-macrophage activation may promote RVF in PAH.
Objectives: Evaluating the contribution of the NLRP3 inflammasome in RV macrophages to PAH RVF.
Methods: Rats with decompensated RV hypertrophy (monocrotaline [MCT] and Sugen-5416 hypoxia [SuHx]) were compared with compensated RV hypertrophy rats (pulmonary artery banding). Echocardiography and right heart catheterization were performed. Macrophages, atrial natriuretic peptides, and fibrosis were evaluated by microscopy or flow cytometry. NLRP3 inflammasome activation and cardiotoxicity were confirmed by immunoblot and in vitro strategies. MCT rats were treated with SC-144 (a GP130 antagonist) or MCC950 (an NLRP3 inhibitor …
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Scholar articles
R Al-Qazazi, PDA Lima, SZ Prisco, F Potus… - American journal of respiratory and critical care …, 2022