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The centrality of values in cross-cultural research has more than doubled over the last three decades. This Special Issue investigates values across cultures and focuses on two main levels: individual and national. At the individual level, values express broad, trans-situational motivational goals, affecting individuals’ interpretation of situations, preferences, choices, and actions. At the national level, values reflect the solutions groups develop in response to existential challenges and relate to the way social institutions function. The authors review the role of values at each level and present eight articles included in the special issue, showing the value of values in cross-cultural research.