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PURPOSE: To compare changes in EI using different areas of interest (small box, large box and muscle trace) immediately following naïve exposure to exercise as well as 24 and 48 hours following an exercise bout.

METHODS: 20 non-resistance trained individuals visited the laboratory 4 times. During visit 1, paperwork and maximum strength were measured. During visit 2 (2-7 days later) participant’s muscle thickness was measured in both arms before performing 4 sets of biceps curls. Additional measures of muscle thickness were taken immediately after exercise, as well as 24 and 48 hours after following the exercise bout. EI of muscle thickness images was measured using three different techniques: 1) Trace around the muscle to include the entire musculature, without any surrounding tissue; 2) Small box placed in the middle of the muscle (2 x 2 cm); 3) Maximal rectangular box that includes as much muscle as …