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This study compared test–retest reliability and sensitivity to change for muscle size and echo intensity (EI) measurements from single transverse (EI_ST) versus panoramic (EI_P) ultrasound (US) images of the biceps brachii. Forearm flexor muscle thickness and EI_ST and biceps brachii muscle cross-sectional area and EI_P were quantified from single transverse and panoramic US images in 14 men (age = 21.8 ± 2.5 y [mean ± standard deviation]) on two separate days. The intra-class correlation coefficients, coefficients of variation, and minimum differences for muscle thickness, EI_ST, muscle cross-sectional area and EIP ranged from 0.78 to 0.99, from 2.26% to 3.29%, and from 6.26% to 9.12%, respectively. These findings suggested that single transverse imaging and panoramic US imaging are comparable, reliable techniques for quantifying muscle size and EI of the biceps brachii. Single transverse images may be …