A versatile ligation-independent cloning method suitable for high-throughput expression screening applications NS Berrow, D Alderton, S Sainsbury, J Nettleship, R Assenberg, ... Nucleic acids research 35 (6), e45, 2007 | 643 | 2007 |
Lysine methylation as a routine rescue strategy for protein crystallization TS Walter, C Meier, R Assenberg, KF Au, J Ren, A Verma, JE Nettleship, ... Structure 14 (11), 1617-1622, 2006 | 366 | 2006 |
Glycoprotein structural genomics: solving the glycosylation problem VT Chang, M Crispin, AR Aricescu, DJ Harvey, JE Nettleship, JA Fennelly, ... Structure 15 (3), 267-273, 2007 | 347 | 2007 |
The nsp9 replicase protein of SARS-coronavirus, structure and functional insights G Sutton, E Fry, L Carter, S Sainsbury, T Walter, J Nettleship, N Berrow, ... Structure 12 (2), 341-353, 2004 | 303 | 2004 |
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications C Tiede, AAS Tang, SE Deacon, U Mandal, JE Nettleship, RL Owen, ... Protein Engineering, Design and Selection 27 (5), 145-155, 2014 | 222 | 2014 |
Affimer proteins are versatile and renewable affinity reagents C Tiede, R Bedford, SJ Heseltine, G Smith, I Wijetunga, R Ross, ... eLife 6, 2017 | 213 | 2017 |
Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multiangle or static light scattering JE Nettleship, J Brown, MR Groves, A Geerlof Structural Proteomics: High-throughput Methods, 299-318, 2008 | 173 | 2008 |
In situ macromolecular crystallography using microbeams D Axford, RL Owen, J Aishima, J Foadi, AW Morgan, JI Robinson, ... Acta Crystallographica Section D: Biological Crystallography 68 (5), 592-600, 2012 | 147 | 2012 |
Recent advances in the production of proteins in insect and mammalian cells for structural biology JE Nettleship, R Assenberg, JM Diprose, N Rahman-Huq, RJ Owens Journal of structural biology 172 (1), 55-65, 2010 | 131 | 2010 |
Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor FcɛRI MD Holdom, AM Davies, JE Nettleship, SC Bagby, B Dhaliwal, E Girardi, ... Nature Structural and Molecular Biology 18 (5), 571, 2011 | 122 | 2011 |
The impact of protein characterization in structural proteomics A Geerlof, J Brown, B Coutard, MP Egloff, FJ Enguita, MJ Fogg, ... Acta Crystallographica Section D: Biological Crystallography 62 (10), 1125-1136, 2006 | 121 | 2006 |
Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling TA Bowden, AR Aricescu, JE Nettleship, C Siebold, N Rahman-Huq, ... Structure 17 (10), 1386-1397, 2009 | 114 | 2009 |
Outrunning free radicals in room-temperature macromolecular crystallography RL Owen, D Axford, JE Nettleship, RJ Owens, JI Robinson, AW Morgan, ... Acta Crystallographica Section D: Biological Crystallography 68 (7), 810-818, 2012 | 96 | 2012 |
ENPP1 processes protein ADP‐ribosylation in vitro L Palazzo, CM Daniels, JE Nettleship, N Rahman, RL McPherson, ... The FEBS journal 283 (18), 3371-3388, 2016 | 78 | 2016 |
The production of glycoproteins by transient expression in mammalian cells JE Nettleship, N Rahman-Huq, RJ Owens High Throughput Protein Expression and Purification, 245-263, 2009 | 54 | 2009 |
A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells JE Nettleship, J Ren, N Rahman, NS Berrow, D Hatherley, AN Barclay, ... Protein expression and purification 62 (1), 83-89, 2008 | 49 | 2008 |
Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins JE Nettleship, PJ Watson, N Rahman-Huq, L Fairall, MG Posner, ... Insoluble proteins, 209-222, 2015 | 48 | 2015 |
Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action JI Robinson, EW Baxter, RL Owen, M Thomsen, DC Tomlinson, ... Proceedings of the National Academy of Sciences, 201707856, 2017 | 45 | 2017 |
Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway NH Tomczyk, JE Nettleship, RL Baxter, HJ Crichton, SP Webster, ... FEBS letters 513 (2-3), 299-304, 2002 | 39 | 2002 |
The structure of a reduced form of OxyR from Neisseria meningitidis S Sainsbury, J Ren, JE Nettleship, NJ Saunders, DI Stuart, RJ Owens BMC structural biology 10 (1), 10, 2010 | 36 | 2010 |