Improvement of enzyme activity, stability and selectivity via immobilization techniques C Mateo, JM Palomo, G Fernandez-Lorente, JM Guisan, ... Enzyme and microbial technology 40 (6), 1451-1463, 2007 | 3876 | 2007 |
Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes JM Guisán Enzyme and Microbial Technology 10 (6), 375-382, 1988 | 707 | 1988 |
Immobilization of enzymes and cells JM Guisan Humana Press, 2006 | 701 | 2006 |
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports A Bastida, P Sabuquillo, P Armisen, R Fernández‐Lafuente, J Huguet, ... Biotechnology and bioengineering 58 (5), 486-493, 1998 | 646 | 1998 |
Immobilization of lipases by selective adsorption on hydrophobic supports R Fernandez-Lafuente, P Armisén, P Sabuquillo, G Fernández-Lorente, ... Chemistry and physics of lipids 93 (1-2), 185-197, 1998 | 610 | 1998 |
Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions L Betancor, F López-Gallego, A Hidalgo, N Alonso-Morales, GDOC Mateo, ... Enzyme and microbial technology 39 (4), 877-882, 2006 | 501 | 2006 |
Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases JM Palomo, G Muñoz, G Fernández-Lorente, C Mateo, ... Journal of Molecular Catalysis B: Enzymatic 19, 279-286, 2002 | 487 | 2002 |
Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins C Mateo, JM Palomo, M Fuentes, L Betancor, V Grazu, F López-Gallego, ... Enzyme and Microbial Technology 39 (2), 274-280, 2006 | 455 | 2006 |
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆ C Mateo, O Abian, R Fernandez–Lafuente, JM Guisan Enzyme and Microbial Technology 26 (7), 509-515, 2000 | 435 | 2000 |
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ... Biotechnology progress 18 (3), 629-634, 2002 | 405 | 2002 |
Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their … C Mateo, G Fernández-Lorente, O Abian, R Fernández-Lafuente, ... Biomacromolecules 1 (4), 739-745, 2000 | 395 | 2000 |
Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports F López-Gallego, L Betancor, C Mateo, A Hidalgo, N Alonso-Morales, ... Journal of Biotechnology 119 (1), 70-75, 2005 | 382 | 2005 |
Immobilization of enzymes on heterofunctional epoxy supports C Mateo, V Grazu, JM Palomo, F Lopez-Gallego, R Fernandez-Lafuente, ... Nature Protocols 2 (5), 1022-1033, 2007 | 344 | 2007 |
Some special features of glyoxyl supports to immobilize proteins C Mateo, O Abian, M Bernedo, E Cuenca, M Fuentes, ... Enzyme and Microbial Technology 37 (4), 456-462, 2005 | 343 | 2005 |
Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method R Fernandez-Lafuente, CM Rosell, V Rodríguez, C Santana, G Soler, ... Enzyme and Microbial Technology 15 (7), 546-550, 1993 | 317 | 1993 |
Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support–polyethylenimine composites C Mateo, O Abian, R Fernandez‐Lafuente, JM Guisan Biotechnology and Bioengineering 68 (1), 98-105, 2000 | 302 | 2000 |
Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method C Mateo, R Torres, G Fernández-Lorente, C Ortiz, M Fuentes, A Hidalgo, ... Biomacromolecules 4 (3), 772-777, 2003 | 300 | 2003 |
General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality JM Palomo, M Fuentes, G Fernández-Lorente, C Mateo, JM Guisan, ... Biomacromolecules 4 (1), 1-6, 2003 | 276 | 2003 |
Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: correlation between enzyme … J Pedroche, M del Mar Yust, C Mateo, R Fernández-Lafuente, ... Enzyme and Microbial Technology 40 (5), 1160-1166, 2007 | 260 | 2007 |
Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment RM Blanco, JJ Calvete, JM Guisán Enzyme and microbial technology 11 (6), 353-359, 1989 | 252 | 1989 |