Engineering of protein secretion in yeast: strategies and impact on protein production A Idiris, H Tohda, H Kumagai, K Takegawa Applied microbiology and biotechnology 86, 403-417, 2010 | 416 | 2010 |
Molecular Characterization and Heterologous Expression of the Gene Encoding a Low-Molecular-Mass Endoglucanase from Trichoderma reesei QM9414 H Okada, K Tada, T Sekiya, K Yokoyama, A Takahashi, H Tohda, ... Applied and Environmental Microbiology 64 (2), 555-563, 1998 | 178 | 1998 |
Phosphorothioate-containing RNAs show mRNA activity in the prokaryotic translation systems in vitro T Ueda, H Tohda, N Chikazumi, F Eckstein, K Watanabe Nucleic acids research 19 (3), 547-552, 1991 | 140 | 1991 |
Zinc finger protein Prz1 regulates Ca2+ but not Cl− homeostasis in fission yeast: identification of distinct branches of calcineurin signaling pathway in fission yeast S Hirayama, R Sugiura, Y Lu, T Maeda, K Kawagishi, M Yokoyama, ... Journal of Biological Chemistry 278 (20), 18078-18084, 2003 | 99 | 2003 |
Schizosaccharomyces pombe minimum genome factory Y Giga‐Hama, H Tohda, K Takegawa, H Kumagai Biotechnology and Applied Biochemistry 46 (3), 147-155, 2007 | 86 | 2007 |
Production of heterologous proteins using the fission‐yeast (Schizosaccharomyces pombe) expression system K Takegawa, H Tohda, M Sasaki, A Idiris, T Ohashi, H Mukaiyama, ... Biotechnology and applied biochemistry 53 (4), 227-235, 2009 | 75 | 2009 |
Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway A Idiris, H Tohda, M Sasaki, K Okada, H Kumagai, Y Giga-Hama, ... Applied microbiology and biotechnology 85, 667-677, 2010 | 67 | 2010 |
Atf1 is a target of the mitogen-activated protein kinase Pmk1 and regulates cell integrity in fission yeast H Takada, M Nishimura, Y Asayama, Y Mannse, S Ishiwata, A Kita, A Doi, ... Molecular biology of the cell 18 (12), 4794-4802, 2007 | 67 | 2007 |
Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomyces pombe and characterization of its products H Okada, T Sekiya, K Yokoyama, H Tohda, H Kumagai, Y Morikawa Applied microbiology and biotechnology 49, 301-308, 1998 | 67 | 1998 |
The gld1 + gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe T Matsuzawa, T Ohashi, A Hosomi, N Tanaka, H Tohda, K Takegawa Applied microbiology and biotechnology 87, 715-727, 2010 | 66 | 2010 |
High–Level Expression of Human Lipocortin I in the Fission Yeast Schizosaccharomyces pombe Using a Novel Expression Vector Y Giga-Hama, H Tohda, H Okada, MK Owada, H Okayama, H Kumagai Bio/technology 12 (4), 400-404, 1994 | 66 | 1994 |
A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast K Hirashima, T Iwaki, K Takegawa, Y Giga-Hama, H Tohda Nucleic acids research 34 (2), e11-e11, 2006 | 63 | 2006 |
Enhanced productivity of protease-sensitive heterologous proteins by disruption of multiple protease genes in the fission yeast Schizosaccharomyces pombe A Idiris, H Tohda, K Bi, A Isoai, H Kumagai, Y Giga-Hama Applied microbiology and biotechnology 73, 404-420, 2006 | 57 | 2006 |
Construction of a protease‐deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease‐sensitive heterologous … A Idiris, K Bi, H Tohda, H Kumagai, Y Giga‐Hama Yeast 23 (2), 83-99, 2006 | 56 | 2006 |
A human papillomavirus type 16 vaccine by oral delivery of L1 protein T Sasagawa, M Tani, W Basha, RC Rose, H Tohda, Y Giga-Hama, ... Virus Research 110 (1-2), 81-90, 2005 | 51 | 2005 |
Novel carbohydrate specificity of the 16-kDa galectin from Caenorhabditis elegans: binding to blood group precursor oligosaccharides (type 1, type 2, Tα, and Tβ … H Ahmed, MA Bianchet, LM Amzel, J Hirabayashi, K Kasai, Y Giga-Hama, ... Glycobiology 12 (8), 451-461, 2002 | 48 | 2002 |
Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe T Matsuzawa, Y Fujita, H Tohda, K Takegawa Eukaryotic cell 11 (2), 159-167, 2012 | 47 | 2012 |
Identification of a galactose‐specific flocculin essential for non‐sexual flocculation and filamentous growth in Schizosaccharomyces pombe T Matsuzawa, T Morita, N Tanaka, H Tohda, K Takegawa Molecular microbiology 82 (6), 1531-1544, 2011 | 45 | 2011 |
Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast R Satoh, T Morita, H Takada, A Kita, S Ishiwata, A Doi, K Hagihara, ... Molecular biology of the cell 20 (9), 2473-2485, 2009 | 43 | 2009 |
Production of D‐amino acid oxidase (DAO) of Trigonopsis variabilis in Schizosaccharomyces pombe and the characterization of biocatalysts prepared with … A Isoai, H Kimura, A Reichert, K Schörgendorfer, K Nikaido, H Tohda, ... Biotechnology and Bioengineering 80 (1), 22-32, 2002 | 41 | 2002 |